The Presbytery of London is made up of 29 churches in 22 pastoral charges. Information about each congregation is provided below. Additional information about a congregation can be acquired by visiting the church's home page or contacting the church office.

		Almanarah Presbyterian Church 250 Hamilton Rd. East London, Ontario
		Appin Presbyterian Church 54 Wellington Street Appin, Ontario
		Argyle Presbyterian Church Highway 76, North of West Lorne
		Bethel Presbyterian Church Richmond Street at Ilderton Road Ilderton, Ontario
		Burns Presbyterian Church 24493 Dundonald Road Glencoe, Ontario
		Chalmers Presbyterian Church 342 Pond Mills Road London, Ontario
		DaySpring Presbyterian Church 1880 Phillbrook Drive London, Ontario
		Dorchester Presbyterian Church 1669 Richmond Street Dorchester, Ontario
		Duff Largie Presbyterian Church County Rd. 9, North of Dutton
		Elmwood Avenue Presbyterian Church 111 Elmwood Avenue East London, Ontario
		Glencoe Presbyterian Church 177 Main Street Glencoe, Ontario
		Guthrie Presbyterian Church 606 Brook Street Melbourne, Ontario
		Knollwood Presbyterian Church 977 Oxford Street East London, Ontario
		Knox Presbyterian Church Fingal, Ontario
		Knox Presbyterian Church Komoka, Ontario
		Knox-St. Andrew's Church Shackleton Street Dutton, Ontario
		Knox Presbyterian Church 55 Hincks Street St. Thomas, Ontario
		New Saint James Presbyterian Church 280 Oxford Street East London, Ontario
		North Caradoc Saint Andrew's Presbyterian Church 8503 Scotchmere Drive, R.R. #1 Strathroy, Ontario
		Oakridge Presbyterian Church 970 Oxford Street W London, Ontario
		Saint Georges Presbyterian Church 1475 Dundas Street East London, Ontario
		St. James Presbyterian Church 44936 Ferguson Line Municipality of Central Elgin, Ontario
		St. John's Presbyterian Church, Port Stanley 238 Colborne Street Port Stanley, Ontario
		Saint John's Presbyterian Church, Wardsville Wardsville, Ontario
		Saint Lawrence Presbyterian Church 910 Huron Street London, Ontario
		South Nissouri Presbyterian Church 17358 Oliver Road West Nissouri, Ontario
		Trinity Presbyterian Church 590 Gainsborough Road London, Ontario
		Westmount Presbyterian Church 521 Village Green Avenue London, Ontario